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source 15s  (Cytiva Europe)


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    Structured Review

    Cytiva Europe source 15s
    Source 15s, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 95/100, based on 305 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/source+15s/SOURCE+15S/custom%4017094401%4042728799
    Average 95 stars, based on 305 article reviews
    source 15s - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Expressing:

    Article Title: Conserved mRNP remodeling mechanism of the TREX-2.1 complex
    Article Snippet: SF, and 2 mg/l aprotinin. The scTREX-2.1 complex was subjected to tandem affinity purification, first with Ni Sepharose 6 fast flow resin (Cytiva) and subsequently with Glutathione Sepharose 4B resin (Cytiva) to obtain a stoichiometric complex. Next, expression tags were cleaved with GST-TEV at 4°C overnight. Digested protein was purified on a Source 15S column (Cytiva) using a linear gradient of N

    Affinity Purification:

    Article Title: Conserved mRNP remodeling mechanism of the TREX-2.1 complex
    Article Snippet: SF, and 2 mg/l aprotinin. The scTREX-2.1 complex was subjected to tandem affinity purification, first with Ni Sepharose 6 fast flow resin (Cytiva) and subsequently with Glutathione Sepharose 4B resin (Cytiva) to obtain a stoichiometric complex. Next, expression tags were cleaved with GST-TEV at 4°C overnight. Digested protein was purified on a Source 15S column (Cytiva) using a linear gradient of N

    Purification:

    Article Title: Conserved mRNP remodeling mechanism of the TREX-2.1 complex
    Article Snippet: SF, and 2 mg/l aprotinin. The scTREX-2.1 complex was subjected to tandem affinity purification, first with Ni Sepharose 6 fast flow resin (Cytiva) and subsequently with Glutathione Sepharose 4B resin (Cytiva) to obtain a stoichiometric complex. Next, expression tags were cleaved with GST-TEV at 4°C overnight. Digested protein was purified on a Source 15S column (Cytiva) using a linear gradient of N

    Nucleic Acid Electrophoresis:

    Article Title: Conserved mRNP remodeling mechanism of the TREX-2.1 complex
    Article Snippet: SF, and 2 mg/l aprotinin. The scTREX-2.1 complex was subjected to tandem affinity purification, first with Ni Sepharose 6 fast flow resin (Cytiva) and subsequently with Glutathione Sepharose 4B resin (Cytiva) to obtain a stoichiometric complex. Next, expression tags were cleaved with GST-TEV at 4°C overnight. Digested protein was purified on a Source 15S column (Cytiva) using a linear gradient of N

    Electrophoresis:

    Article Title: Conserved mRNP remodeling mechanism of the TREX-2.1 complex
    Article Snippet: SF, and 2 mg/l aprotinin. The scTREX-2.1 complex was subjected to tandem affinity purification, first with Ni Sepharose 6 fast flow resin (Cytiva) and subsequently with Glutathione Sepharose 4B resin (Cytiva) to obtain a stoichiometric complex. Next, expression tags were cleaved with GST-TEV at 4°C overnight. Digested protein was purified on a Source 15S column (Cytiva) using a linear gradient of N

    Polyacrylamide Gel Electrophoresis:

    Article Title: Conserved mRNP remodeling mechanism of the TREX-2.1 complex
    Article Snippet: SF, and 2 mg/l aprotinin. The scTREX-2.1 complex was subjected to tandem affinity purification, first with Ni Sepharose 6 fast flow resin (Cytiva) and subsequently with Glutathione Sepharose 4B resin (Cytiva) to obtain a stoichiometric complex. Next, expression tags were cleaved with GST-TEV at 4°C overnight. Digested protein was purified on a Source 15S column (Cytiva) using a linear gradient of N

    Activity Assay:

    Article Title: Conserved mRNP remodeling mechanism of the TREX-2.1 complex
    Article Snippet: SF, and 2 mg/l aprotinin. The scTREX-2.1 complex was subjected to tandem affinity purification, first with Ni Sepharose 6 fast flow resin (Cytiva) and subsequently with Glutathione Sepharose 4B resin (Cytiva) to obtain a stoichiometric complex. Next, expression tags were cleaved with GST-TEV at 4°C overnight. Digested protein was purified on a Source 15S column (Cytiva) using a linear gradient of N

    Lysis:

    Article Title: Conserved mRNP remodeling mechanism of the TREX-2.1 complex
    Article Snippet: SF, and 2 mg/l aprotinin. The scTREX-2.1 complex was subjected to tandem affinity purification, first with Ni Sepharose 6 fast flow resin (Cytiva) and subsequently with Glutathione Sepharose 4B resin (Cytiva) to obtain a stoichiometric complex. Next, expression tags were cleaved with GST-TEV at 4°C overnight. Digested protein was purified on a Source 15S column (Cytiva) using a linear gradient of N

    Centrifugation:

    Article Title: Conserved mRNP remodeling mechanism of the TREX-2.1 complex
    Article Snippet: SF, and 2 mg/l aprotinin. The scTREX-2.1 complex was subjected to tandem affinity purification, first with Ni Sepharose 6 fast flow resin (Cytiva) and subsequently with Glutathione Sepharose 4B resin (Cytiva) to obtain a stoichiometric complex. Next, expression tags were cleaved with GST-TEV at 4°C overnight. Digested protein was purified on a Source 15S column (Cytiva) using a linear gradient of N

    Chromatography:

    Article Title: Conserved mRNP remodeling mechanism of the TREX-2.1 complex
    Article Snippet: SF, and 2 mg/l aprotinin. The scTREX-2.1 complex was subjected to tandem affinity purification, first with Ni Sepharose 6 fast flow resin (Cytiva) and subsequently with Glutathione Sepharose 4B resin (Cytiva) to obtain a stoichiometric complex. Next, expression tags were cleaved with GST-TEV at 4°C overnight. Digested protein was purified on a Source 15S column (Cytiva) using a linear gradient of N

    Fast Protein Liquid Chromatography:

    Article Title: Conserved mRNP remodeling mechanism of the TREX-2.1 complex
    Article Snippet: SF, and 2 mg/l aprotinin. The scTREX-2.1 complex was subjected to tandem affinity purification, first with Ni Sepharose 6 fast flow resin (Cytiva) and subsequently with Glutathione Sepharose 4B resin (Cytiva) to obtain a stoichiometric complex. Next, expression tags were cleaved with GST-TEV at 4°C overnight. Digested protein was purified on a Source 15S column (Cytiva) using a linear gradient of N

    High Performance Liquid Chromatography:

    Article Title: Conserved mRNP remodeling mechanism of the TREX-2.1 complex
    Article Snippet: SF, and 2 mg/l aprotinin. The scTREX-2.1 complex was subjected to tandem affinity purification, first with Ni Sepharose 6 fast flow resin (Cytiva) and subsequently with Glutathione Sepharose 4B resin (Cytiva) to obtain a stoichiometric complex. Next, expression tags were cleaved with GST-TEV at 4°C overnight. Digested protein was purified on a Source 15S column (Cytiva) using a linear gradient of N

    Crystallization Assay:

    Article Title: Conserved mRNP remodeling mechanism of the TREX-2.1 complex
    Article Snippet: SF, and 2 mg/l aprotinin. The scTREX-2.1 complex was subjected to tandem affinity purification, first with Ni Sepharose 6 fast flow resin (Cytiva) and subsequently with Glutathione Sepharose 4B resin (Cytiva) to obtain a stoichiometric complex. Next, expression tags were cleaved with GST-TEV at 4°C overnight. Digested protein was purified on a Source 15S column (Cytiva) using a linear gradient of N



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    Image Search Results


    Journal: Molecular cell

    Article Title: BRD4 binds the nucleosome via both histone and DNA interactions

    doi: 10.1016/j.molcel.2026.07.012

    Figure Lengend Snippet:

    Article Snippet: Histone complexes were further purified by Source S cation-exchange chromatography (GE Healthcare, Cat#17–0944-01).

    Techniques: Virus, Recombinant, Plasmid Preparation, Sequencing, Software, Membrane